My list:

(1) Green Goblin
(2) Doctor Octopus
(3) Lizard
(4) Kingpin
(5) Kraven the Hunter
(6) Vulture(s)
(7) Mysterio
(8) Sandman
(9) Spencer Smythe & Spider-Slayers
(10) Scorpion
(11) Chameleon
(12) Electro
(13) Rhino
(14) Enforcers
(15) Beetle
(16) Morbius
(17) Silvermane
(18) Hammerhead
(19) Molten Man
(20) Shocker

What would be yours?

I recognize all the names in your list, so you must be an old-school reader like me. I haven't bought a contemporary Spider-man comic in at least two decades.

Plus, I don't think I've ever stopped to consider Top 20. Maybe Top 5. But, my list just the same:

1. Green Goblin
2. Sandman
3. The Rhino
4. Electro
5. The Scorpion
6. Dr. Octopus
7. The Fly
8. The Beetle
9. The Shocker
10. The Molten Man
11. The Tarantula
12. The Vulture
13. Hydroman
14. Hobgoblin
15. Venom
16. Boomerang
17. The Grizzly
18. Equinox (since Marvel Team-up was Peter's title for so long, the enemies introduced there should be considered his rogues).
19. Stegron
20. The Lizard

I started reading comics in the 1980s. (^_^)

I am fond of the old-school villains. They awake my imagination.



In my case, on and off.



I agree.