Previous Post (1) The Vulture https://marvel.fandom.com/wiki/Amazing_Spider-Man_Vol_1_48 (2) The Green Goblin https://marvel.fandom.com/wiki/Amazing_Spider-Man_Vol_1_122 (3) Doctor Octopus https://marvel.fandom.com/wiki/Amazing_Spider-Man_Vol_1_131 https://marvel.fandom.com/wiki/Spectacular_Spider-Man_Vol_1_221 (4) Mysterio https://marvel.fandom.com/wiki/Amazing_Spider-Man_Vol_1_141 https://marvel.fandom.com/wiki/Daredevil_Vol_2_7 (5) Kraven the Hunter https://marvel.fandom.com/wiki/Amazing_Spider-Man_Vol_1_294 (6) The Lizard https://marvel.fandom.com/wiki/Web_of_Spider-Man_Vol_1_111 Nota Bene: it also happened to other Spidey villains (notably, Chameleon, Rhino & Electro).

(1) Green Goblin
The Night Gwen Stacy Died
https://marvel.fandom.com/wiki/The_Night_Gwen_Stacy_Died
SSM Vol 1 #199-200 (Harry Osborn, Green Goblin 2)
https://marvel.fandom.com/wiki/Spectacular_Spider-Man_Vol_1_199

(2) Kraven the Hunter
Kraven's Last Hunt
https://marvel.fandom.com/wiki/Kraven's_Last_Hunt
Hunted
https://marvel.fandom.com/wiki/Hunted_(Spider-Man_Story_Arc)

(3) Doctor Octopus
Web of Death
https://marvel.fandom.com/wiki/Web_of_Death
ASM Vol 1 #600-700:
- Last Legs
https://marvel.fandom.com/wiki/Amazing_Spider-Man_Vol_1_600#Appearing_in_.22Last_Legs.22
- Origin of Species
https://marvel.fandom.com/wiki/Amazing_Spider-Man_Vol_1_642
- Ends of the Earth
https://marvel.fandom.com/wiki/Ends_of_the_Earth
- Dying Wish
https://marvel.fandom.com/wiki/Dying_Wish

(4) Mysterio
Guardian Devil
https://marvel.fandom.com/wiki/Guardian_Devil

(5) The Vulture
Funeral Arrangements
https://marvel.fandom.com/wiki/Funeral_Arrangements

(6) The Lizard
The Savaging
https://marvel.fandom.com/wiki/The_Savaging


Nota Bene:
There is also the swan song of another Spidey villain, Spencer Smythe.
https://marvel.fandom.com/wiki/Amazing_Spider-Man_Vol_1_191